Coding

Part:BBa_K2050421:Design

Designed by: Muhammad Farhan Maruli   Group: iGEM16_UI-Indonesia   (2016-10-13)


Thosea Asigna Virus 2A peptide linker (T2A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence was optimized for human expression to achieve maximum codon adaptation index possible (evaluated with GenScript's rare codon analysis tool) with considerations to its flanking sequences.


Source

We looked up the amino acid sequence in Yang et al. (doi:10.1038/gt.2008.90) and Tang et al. (10.1523/JNEUROSCI.0359-09.2009), and then created the DNA sequence ourselves.

References